- we immunize
mice with any desired antigen to produce hybridomas. We
screen for specificity, affinity, efficacy or any combination.
- We
fuse primary B cells from mice with mice myelomas and screen
for specificity, affinity, efficacy or any combination.
- We isolate heavy and light chain variable regions clone
variable regions. These regions are cloned into any desired
expression vector.
- We genetically fuse (light or heavy chain) variable regions
with a human constant region of any sub-class.
- We produce polymeric antibodies with properties of a single
antibody or properties of different sub-classes. For example,
we can construct an IgG polymeric with the properties of
an IgA or IgM antibody.
- We produce antibodies with two different variable regions
specific for two different target antigens.
- We genetically fuse antibodies or antibody fragments to
non-antibody proteins to increase their effectiveness as
diagnostic or therapeutic agents. For example, antibodies
can be genetically fused to cytokines, toxins or any other
protein.
- We grow hybridomas in large cultures. We purify monoclonal
antibodies from hybridoma supernatants.
-- We grow transfectants in large cultures. We purify recombinant
antibodies from transfectoma supernatants.
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