Execution

Immunize mice - we immunize mice with any desired antigen to produce hybridomas. We screen for specificity, affinity, efficacy or any combination.

Produce hybridomas - We fuse primary B cells from mice with mice myelomas and screen for specificity, affinity, efficacy or any combination.

Isolate variable region genes - We isolate heavy and light chain variable regions clone variable regions. These regions are cloned into any desired expression vector.

Produce chimeric antibodies - We genetically fuse (light or heavy chain) variable regions with a human constant region of any sub-class.

Produce polymeric antibodies - We produce polymeric antibodies with properties of a single antibody or properties of different sub-classes. For example, we can construct an IgG polymeric with the properties of an IgA or IgM antibody.

Produce Bispecific antibodies - We produce antibodies with two different variable regions specific for two different target antigens.

Produce antibody fusion proteins - We genetically fuse antibodies or antibody fragments to non-antibody proteins to increase their effectiveness as diagnostic or therapeutic agents. For example, antibodies can be genetically fused to cytokines, toxins or any other protein.

Purify monoclonal antibodies - We grow hybridomas in large cultures. We purify monoclonal antibodies from hybridoma supernatants.

Purify recombinant proteins -- We grow transfectants in large cultures. We purify recombinant antibodies from transfectoma supernatants.

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